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The merged image (yellow) shows the same JG cell labeled with both antibodies. Detection of ET receptors in freshly fixed mouse renal cortical slices Figure 3 represents freshly fixed renal cortical sections labeled for the ETA receptor (on the top) and cortical sections labeled for the ETB receptor (on the bottom). ET receptors (both ETA and ETB) are expressed throughout the proximal tubule as expected (Kohan et al. 2011), as well as the afferent arteriole (aa) next to the glomerulus (G), The immunolabeling demonstrates that [http://www.selleckchem.com/products/17-AAG(Geldanamycin).html Tanespimycin] both ETA and ETB receptors are expressed in the afferent arteriole (Barajas 1979) in mouse renal cortical slices fixed in vivo consistent with labeling in isolated primary cultures of JG cells. Figure 3. Immunoflourescence of mouse renal cortical sections fixed in situ showing (in red) ET receptors A (top) and ETB (bottom) in the afferent arteriole (aa) within the juxtaglomerular apparatus next to the glomerulus (G) using antibodies selective for each ... RT�\PCR for TRPC1/4/5 Figure 4 shows that the RT�\PCR performed on 1 ��g of total RNA gave positive products for TRPC1, 4, and 5 at the expected size 238, 168 (Fig. ?(Fig.44 top), and 247 bp (Fig. ?(Fig.44 bottom), respectively. The ��no�\template�� negative control showed no amplification. [http://www.selleckchem.com/products/Pazopanib-Hydrochloride.html check details] These results suggest that there is expression of TRPC1 and TRPC4 and TRPC5 in JG cells. Figure 4. Two different gels; one for TRPC1 and TRPC4 (top) and another for TRPC5 (bottom) isoforms, run under the same conditions. RT�\PCR for TRPC 1, 4, and 5 identifying three bands at a 238, 168, and 247 bp. On the top columns 1 and 2 total mRNA obtained ... Endothelin 1 and 2 inhibit renin release Incubation of JG cells with 1 nmol/L ET�\1 decreased basal renin release by 30% from 0.30 �� 0.04 to 0.23 �� 0.03 ��gAngI/mL/h/mg prot. Incubation of JG cells with 10 nmol/L ET�\1 likewise decreased renin release to 0.20 �� 0.02 ��gAngI/mL/h/mg prot. (P [http://en.wikipedia.org/wiki/Adenosine Adenosine] with 10 nmol/L ET�\2 likewise decreased renin release to 0.24 �� 0.01 ��gAngI/mL/h/mg prot. (P