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Analysis revealed that aging liver-hfer mice, exposed to chronic elevated expression of h-ferritin, have increased R2 values compared to WT. As expected for ferritin, R2 difference was strongly enhanced at high magnetic field. Histological analysis of these mice did not reveal liver changes with prolonged over expression of ferritin, and no differences could be detected in other organs. Furthermore, dietary iron supplementation significantly affected MRI contrast, without affecting animal wellbeing, for both wildtype and ferritin over expressing transgenic mice. These results suggest the safety of ferritin over-expression, [http://www.selleckchem.com/products/Y-27632.html Y-27632 purchase] and support the use of h-ferritin as a reporter gene for MRI. Copyright ? 2010 John Wiley & Sons, Ltd. ""The aims of this study were to characterise [http://www.selleckchem.com/products/chir-99021-ct99021-hcl.html CHIR-99021 chemical structure] the major saturated and unsaturated lipid peaks in histologically normal cervical epithelium and stroma, dysplastic epithelium (low-grade cervical intraepithelial neoplasia, CIN) and cancer-containing tissue samples from patients with cervical cancer using diffusion-weighted 1H high-resolution magic angle spinning MRS, to determine whether mobile lipid resonances (MLRs) distinguish tissue types and to test for a correlation between MLRs and the number of cytoplasmic lipid droplets. Diffusion-weighted spectra of tissue biopsies were acquired using a stimulated echo sequence with bipolar gradients. Major saturated and unsaturated MLRs were identified and multivariate analysis of peak combinations was used to determine the best separation between tissue classes. Lipid droplets were visualised with Nile red staining and fluorescence microscopy. Correlations of saturated lipid resonances (0.9 and 1.3?ppm), polyunsaturated resonances (2.8?ppm), triglycerides (4.3?ppm) and unsaturated resonances (5.3?ppm) with average droplet number (per image) were investigated using a Spearman rank test. A large heterogeneity in lipid content among samples was observed, resulting in no significant differences in MLR intensities of individual peaks between the three tissue classes. Linear discriminant analysis separated ��no cancer�� from ��cancer�� based on the intensities at 0.9, 1.3, [https://en.wikipedia.org/wiki/Fleroxacin Fleroxacin] 2.2 and 2.8?ppm [area under the curve (AUC)?=?0.939, p?