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05). IgG2a isotype kinetics also showed higher IgG2a levels for the NLA?+?ArtinM group from 15 to 45 d.a.i. when compared to the other groups, with similar IgG2a levels between NLA?+?JAC and NLA groups at 30 and 45 d.a.i. ( Fig. 1C). All control groups showed IgG, IgG1 and IgG2a levels below the cut off. N. caninum immunostaining showed a brighter linear peripheral fluorescence of parasite surfaces when probed with sera from mice immunized with NLA?+?ArtinM in relation to NLA?+?JAC and NLA groups ( Fig. 2). The control group (PBS) showed no staining of tachyzoites. Serological results determined at 60 days after immunization before challenge (BC) and 30 days after challenge (AC) with 2?��?107 tachyzoites of Nc-1 isolate. N. caninum-specific IgG1 and IgG2a isotypes were compared before challenge Selleck GSK2656157 17-DMAG (Alvespimycin) HCl (60 d.a.i.) and 30 days after challenge (90 d.a.i.) with virulent parasite in all experimental groups, including the assay of seroconversion for the control groups ( Fig. 3A). Levels of IgG1 were higher than IgG2a in all antigen-immunized groups regardless of the lectin adjuvant in both conditions, before and after parasite challenge, while a seroconversion with predominant IgG2a response was observed after parasite challenge only in the lectin-immunized groups, but with significant difference for ArtinM lectin alone (P?I-BET151 nmr of stimulation with medium, ConA or NLA (Fig. 4A and B). After antigen stimulation, IFN-�� levels were higher in the NLA?+?ArtinM group in relation to all others (P?