To determine the relevance of IGF-IR in our model of brain metastasis, we first characterized the activation profile of IGF-IR in parental MDA-MB-231 (231P)

To circumvent this issue, we very first immunoprecipitated the IGF-IR 726169-73-9 subunit with a particular antibody that does not cross-react with the insulin receptor, followed by immunoblotting with phospho-IGFIR antibody in opposition to Tyr 1131, the earliest autophosphorylation website that is definitely needed for IGF-one ligand-dependent IGFIR purpose [fifteen]. We found that equally 231Br and BT474Br3 cell traces expressed far more phosphorylated IGF-IR than parental breast cancer cells beneath LY-2484595 manufacturer standard expansion circumstances in full medium (Figure 1C). An average of 36.four% of the 231Br cell population was optimistic for phospho-Tyr1131 IGF-IR, compared to fourteen.6% of 231 parental cells (p