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Differences between individual means were identified using Tukey's studentized range test at the 5 diglyceride % significance level. A non-parametric Kruskall�CWallis test was also used to test differences in glucose and sucrose in buds and fructose, raffinose and starch in buds and stems between cultivars, treatments and harvesting dates. This test was done to supplement the result from the ANOVA, as the assumption concerning similar variance was not fulfilled. The ANOVA and the Kruskall�CWallis test gave similar results when assessing the effects of cultivar, treatment and ST on glucose, sucrose and raffinose in buds and fructose in buds and stems, and only the results of the ANOVA are described. For raffinose in stems and starch in buds and stems, the ANOVA indicated that the contents differed significantly between treatments and cultivars, respectively, whereas the Kruskall�CWallis test did not indicate significant cultivar or treatment differences. Hence, only the results of the Kruskall�CWallis tests are shown. The effects of ST and cultivar on the number of flowers and fruit yield was analysed using two-way ANOVA. The correlation coefficients between LT50 values and air temperatures or concentrations of soluble carbohydrates, and between air temperatures and concentration of soluble carbohydrates were examined using Pearson's correlation coefficient (PROC CORR). Results Climate The autumn of 2012 was relatively mild buy AZD0530 with no freezing events and a daily mean temperature of 6.0 and 6.6 ��C in November at 20 cm height in the control and the warming plot, respectively (Fig.?1A). December, January and February had daily mean temperatures just around the freezing point and absolute minimums of ?9.2 and ?6.7 ��C in the control and warming plots, respectively. The spring of 2013 was unusually cold. In March, the daily mean temperature decreased to ?0.7 ��C in the control plot and 0.3 ��C in the warming plot, and 20 and 16 days had average temperatures below zero. In April and May the temperature increased to on average 6.6 and 12.9 ��C, respectively, in the two plots, but several days in April and 2 days in May still had temperatures below the freezing point, as evidenced GDC-0941 datasheet by the daily minimum air temperature recordings (not shown). Figure?1. Daily mean air (A) and soil (C) temperatures (��C) at 20 cm height or ?15 cm depth, respectively, in the control and warming plots during the experimental period. Also shown are the differences in daily mean temperatures between the warming ... The temperatures measured among the plants were slightly higher than the temperature recordings obtained from the climate station (not shown), but the measurements were otherwise in close agreement. The temperature difference at 20 cm height between the control plot and the warming plot was on average 0.76 ��C throughout the treatment period (Fig.?1B). It varied between