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The samples were coded for Doxorubicin each participant and testing session. Relaxin (hRLX; Immundiagnostik AG, Bensheim, Germany; sensitivity properties over the course of the menstrual cycle were determined using a one-way repeated-measures ANOVA (or Friedman's test where appropriate, i.e. if data were not normally distributed as determined by the Shapiro�CWilk test). Where a significant main effect was present,?post hoc?Student's paired?t?tests were performed and?P-values Bonferroni corrected to examine any differences highlighted. Spearman correlation coefficients were determined to examine the relationship between serum relaxin levels and tendon mechanical properties. Significance was set to?P? coefficients (ICC) were calculated to estimate reliability of the measures. All data are presented as means �� SEM. Within-session ICCs were 0.91 for patellar tendon elongation, 0.945 for medial gastrocnemius tendon elongation, 0.923 for knee extension torque, 0.912 for plantarflexion torque, 0.98 for PTCSA, 0.98 for MGTCSA, 0.92 for PTL, 0.98 for MGTL and 0.98 for relaxin DAPT research buy level. There were no significant differences in the level of serum relaxin, the cross-sectional area of the tendons, or the mechanical properties of either the medial gastrocnemius or patellar tendons across the three phases of the menstrual cycle (P?> 0.05; see Tables 1 and 2 for values). Relaxin concentrations were seen to account for between 26 and 31% of the variation in patellar tendon stiffness. Significant correlations were found between serum relaxin levels and stiffness, for the patellar tendon only (r?=?0.561,?P? 0.05) of the variance in tendon stiffness in the medial gastrocnemius, but these associations were not significant. There were no significant associations between tendon cross-sectional area and relaxin levels (r?=?0.193,?P?> 0.05 in the patellar Sulfatase and?r?=?0.233,?P?> 0.05 in the gastrocnemius tendons). The present study shows that in a group of normally menstruating women (not taking any form of medical contraception), the menstrual cycle impacts neither on relaxin nor on the structural and mechanical properties of the medial gastrocnemius and patella tendons (P?> 0.05). However, a significant relationship (P?