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They were obtained at the apex, middle and base of the left and right prostate lobes in the parasagittal plane. Approximately 10�C12 cores were obtained, depending on the prostate volume. From each topographic zone, a separate core was sent for histopathological investigation. Results were compared by the use of Fisher��s exact test. All tests were two-tailed and were considered significant when p?EX 527 clinical trial prophylaxis with an antibiotic other than a fluoroquinolone (N?=?22) (e.g. co-trimoxazole) were also excluded. In general, patients referred for biopsy by the general practitioner did not receive prophylaxis, and those who received an antibiotic other than a fluoroquinolone were most often treated for a pre-existing Docetaxel ic50 infection. Of the remaining 236 rectal cultures, 178 showed growth on MacConkey agar but not on MacConkey agar with ciprofloxacin 1?mg/L, indicating that these patients harboured ciprofloxacin-sensitive strains. There was growth on both MacConkey agars for 58 rectal cultures. In 52 cultures, the microorganism was identified as E.?coli. The MIC of ciprofloxacin was ��32?mg/L in 34 of 52 (65.4%) E.?coli strains. For the remaining 18 E.?coli strains, the MICs were 4 (N?=?1), 8 (N?=?9), or 16 (N?=?8). For the other six cultures with growth on both MacConkey agars, the microorganisms were identified as Pseudomonas aeruginosa (N?=?2), Comamonas kerstersii (N?=?2), Proteus mirabilis (N?=?1), and Candida albicans (N?=?1). Thus, 22.0% (52/236) of the patients harboured faecal ciprofloxacin-resistant E.?coli strains. Two patient groups, namely those who harboured faecal ciprofloxacin-sensitive strains and those with ciprofloxacin-resistant E.?coli strains, were compared. There was no statistically significant difference in age or prostate-specific antigen level between the groups (Table?1). Only 15 of 178 (8.4%) of patients colonized with fluoroquinolone-susceptible Vatalanib (PTK787) 2HCl E.?coli strains used fluoroquinolones during the 6?months before TRUSPB, in contrast to 20 of 52 (38.5%) of patients colonized with fluoroquinolone-resistant E.?coli strains (p?