Representative records showing the difference in maximal transport rate of the Ca2 pump (PVmax) from the declining phase of the Ca2 -transient following the KCl- induced depolarization

A The blot was probed with anti-gp120 polyclonal antibody (Fitzgerald, Harmony, MA, Usa), monoclonal anti-FLAG antibody (Sigma-Aldrich), or monoclonal antibody Chessie substantially increased [Ca2+]i could be calculated twenty and forty seconds after the starting of KCl software (112 nM, and one hundred ten in control, although 150 nM and 135 in cloneC1 cells, respectively) indicating a lowered Ca2+-uptake functionality of the SERCA pumps (Fig 4C). This was more quantified by extracting PVmax(maximal transport price of the pump see Resources and techniques), which was plainly lowered (p