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Then, with respect to the population of each school and its ratio to the total number of students in that class, some students from each class were selected. At first, 3,000 students (1,500 in middle and 1,500 in high schools) were chosen and http://www.selleckchem.com/products/INCB18424.html a demographic questionnaire together with a consent form was sent to their parents. The parents were requested to fill out the demographic and consent forms and return them to the school if they agree with their child��s participation in the plan. The exclusion criteria were, not having any chronic disease or endocrine disorder such as diabetes and not being on a treatment of corticosteroids or drugs influencing lipid profile. In the next step, after getting the permission from the education office and appropriate coordination, two trained nurses visited the schools to collect and record selleck products the demographic information. Eventually, 2,643 individuals, after the exclusion of a few with incomplete information, were selected for the analysis. To measure blood lipids, including cholesterol, triglyceride, HDL, and LDL, blood samples were derived from the cubital vein of the left hand after a 12 hour fasting. The blood sample was taken in 5 ml vacuum tubes containing separator gel and clot activator manufactured by Bacton Dickinson (UK). The obtained samples were immediately centrifuged and their respective lipid levels were determined by applying enzymatic procedure using German Rosh kits, with Biochemical Autoanalyser Prestige 24i (Japan). Definition of Pediatric Dyslipidemia NHLBI panel definition of dyslipidemia in 2011 was used in our study.4 These cutoff points delineate lipid values as borderline and abnormal were: Borderline: HDL mg/dl and TG>130 mg/dl Abnormal: HDL130 mg/dl The data were analyzed using the SPSS software, version 15. Analysis between categorical variables Megestrol Acetate was assessed by the Chi-square test. The independent t test was used to compare the means between the two groups. While ����0.05 was taken as the significant level. Results In the present study, 2,643 students in the age group of 11-18 with an average 14.5��2 years, including 1,396 (52.5%) girls and 1,247 (47.2%) boys were included (table 1). Most of the students aged 14 (18.7%) and 12-13 years (17.7%). The mean weight, stature, waist circumference, and BMI were measured 49.78��13.22 kg, 156.20��9.68 cm, 68.43��9.75 cm, and 20.21��4.02, respectively. The minimum and maximum of these quantities were determined as 27 and 117 kg, 130 and 187 cm, 47 and 114 cm, and 12.5 and 40.44, respectively. In 904 (34.2%) adolescents, there was at least one lipid disorder (abnormal or borderline) with the order of prevalence in individuals as 42.6% for HDL