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Additional data were collected for all patient groups: age at presentation, sex, duration of illness (>6?months was classified as late LNB), and CSF findings at diagnosis (intrathecal leukocytes and erythrocytes per microlitre, percentage of mononuclear cells, glucose level, total protein, IgG, and albumin). For LNB patients, the clinical presentation, duration of complaints and report of an EM were documented. All sera and CSF samples were tested with the C6 Lyme ELISA Kit (Immunetics, Boston, MA, USA). Preliminary results showed good performance of a 1?:?5 dilution for CSF. Therefore, and for practical reasons, all CSF samples were tested in a 1?:?5 Microtubule Associated inhibitor dilution with the manufacturer��s protocol for serum. C6-peptide ELISA was performed Fluconazole on sera according to the manufacturer��s protocol. The Lyme index (LI) was calculated according to the manufacturer��s protocol: absorbance450�C650?nm sample/[(absorbance450�C650?nm calibrator)?+?0.3]. Samples with LI values 0.150. The AI was considered to be positive when the CSF was positive and the AIIgG or AIIgM was ��0.3. Statistical analysis was performed with a statistical software package (SPSS for Windows, version 17.0). Angiogenesis inhibitor Student��s t-test was used to compare levels of C6-peptide LI between groups, and p-values