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Fig. 4 Average number of transmissions for susceptible clone and PNSP clone by implementing reduced outpatient penicillin consumption. The fitness cost for the PNSP clone was fixed at 5% and the default penicillin consumption was set according Sweden year 2010 ... Further simulations were then performed by implementing 5 and 10% fitness costs, respectively, for the PNSP clone (Fig. 5). The impact of penicillin consumption was examined by implementing outpatient penicillin consumption at three levels; none Decitabine datasheet (no penicillin consumption), low (penicillin consumption according to default volume, Sweden, year 2010; Table 2), and high (doubled penicillin consumption compared with the default volume). A one-way ANOVA Tryptophan synthase showed a significant difference in the number of PNSP transmissions among the simulations performed using 5% fitness cost for the PNSP clone (F2,297=27.9, pPD98059 ic50 high to low level (Fig. 5, p=0.96). However, a statistically significant decrease could be observed when penicillin consumption was reduced from high to none (p