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1 kg by using a digital weighing scale (Seca 841, GmbH & Co., Germany); and body height, to the nearest centimeter by using an extensometer (STDK Model1 HD, Japan). The circumferences of 7 sites (upper arm, flexed upper arm, chest, waist, hip, thigh, and calf) and bone widths (humerus and femur) were measured to the nearest centimeter by using a tape measure and spreading caliper, respectively. The GPCR Compound Library skinfold thickness of 5 sites (triceps, subscapular, suprailiac, thigh, and medial calf) was measured twice on the left side of the body by using a Harpenden caliper (Holtain, Crymych, UK). Body composition Body composition variables were measured by performing a bioelectrical impedance analysis (Inbody 3.0, Transferase Biospace, Korea). Measurements were performed with the participants barefoot and in minimal clothing without any metallic materials. Percent body fat, fat mass, fat-free mass, and total body water were measured. Physical fitness For the physical fitness tests, the European test was used, and 9 variables from 7 factors were tested. Each test was performed according to the following sequence: balance (flamingo balance), speed (plate tapping), flexibility (sit and reach), power (standing long jump), static muscle strength (grip strength), muscular endurance (sit-ups and bent-arm hang), agility (50-m shuttle run), and cardiorespiratory endurance (VO2max). VO2max was determined for each subject during an ��all-out�� progressive exercise test on Vmax 229 (Sensor Medics, USA), according to the Bruce protocol. The VO2max was considered to have been reached if at least 3 of the following criteria were met: (1) when the heart rate does not increase even if the test strength is increased; (2) when the respiratory rate is greater than 1.10%; (3) when the rating of perceived exertion according to Borg��s scale is greater than 17; (4) when heart rate is higher than 90% of the predicted heart rate; and (5) when oxygen consumption is stagnant despite the increase in intensity. Isokinetic strength Isokinetic dynamometry (Cybex 770, NORM, USA) was performed to evaluate flexion and extension of the right and left knees. Each test included 3 maximal contractions at 60��/sec for muscle strength and 25 maximal contractions at 180��/sec for muscle endurance. Statistical analyses Statistical buy Fasudil analyses were performed by using the SAS version 12.0 software for Windows. All data were presented as mean and standard deviation values. Paired t test was used to analyze changes over time in each parameter. P